Honestly for me it was Arsenals game against Norwich at home. We needed them to drop points at some point in the run in and this looked like being it, Norwich were a goal up and looking comfortable, Arsenal looker laboured and had run out of ideas.
Then they get a corner which was clearly off an Arsenal player. I'm thinking to myself 'I bet they'll get something here because of that' and sure enough, the linesman who was 30 yards further away from the incident than the referee who didn't give anything, decides to give a questionable penalty. From that, their tails are up and they get the second, before Walcott makes sure with an offside goal.
They were riding a wave of invincibility following the Munich match and this result could have knocked them out of their stride. I genuinely don't think we had the same kind of lucky call all season whereby a ref just decides to unfairly gift us the match like that. Even if Norwich got the draw we'd be 4th right now.
So it's not a Spurs moment as such but it affected us and was the worst moment of the season for me. I was absolutely furious watching it all unfold.